Androstenedione

At a Glance

Why Get Tested?

To help evaluate adrenal gland function; to detect adrenal tumors or cancers; to help determine the cause of male physical characteristics (virilization) in females or early puberty in boys; to evaluate androgen production and function of ovaries in women or testicular function in men; when congenital adrenal hyperplasia (CAH) is suspected; to monitor treatment for CAH

When To Get Tested?

When a woman has excess facial and body hair (hirsutism), acne, no monthly menstrual periods (amenorrhea), fewer than 6-8 menstrual cycle per year (oligomenorrhea), or infertility; when a boy is undergoing very early (precocious) puberty or a girl is showing signs of virilization; when puberty is delayed; when diagnosing CAH; periodically when being treated for CAH

Sample Required?

A blood sample drawn from a vein in your arm

Test Preparation Needed?

In menstruating women, there may be specific instructions as to timing of the sample collection.

Looking For Test Results?

Looking For Reference Ranges?

You may be able to find your test results on your laboratory’s website or patient portal. However, you are currently at Testing.com. You may have been directed here by your lab’s website in order to provide you with background information about the test(s) you had performed. You will need to return to your lab’s website or portal, or contact your healthcare practitioner in order to obtain your test results.

Testing.com is an award-winning patient education website offering information on laboratory tests. The content on the site, which has been reviewed by laboratory scientists and other medical professionals, provides general explanations of what results might mean for each test listed on the site, such as what a high or low value might suggest to your healthcare practitioner about your health or medical condition.

The reference ranges for your tests can be found on your laboratory report. They are typically found to the right of your results.

If you do not have your lab report, consult your healthcare provider or the laboratory that performed the test(s) to obtain the reference range.

Laboratory test results are not meaningful by themselves. Their meaning comes from comparison to reference ranges. Reference ranges are the values expected for a healthy person. They are sometimes called “normal” values. By comparing your test results with reference values, you and your healthcare provider can see if any of your test results fall outside the range of expected values. Values that are outside expected ranges can provide clues to help identify possible conditions or diseases.

While accuracy of laboratory testing has significantly evolved over the past few decades, some lab-to-lab variability can occur due to differences in testing equipment, chemical reagents, and techniques. This is a reason why so few reference ranges are provided on this site. It is important to know that you must use the range supplied by the laboratory that performed your test to evaluate whether your results are “within normal limits.”

For more information, please read the article Reference Ranges and What They Mean.

What is being tested?

Androstenedione is an androgen, one of several “male” sex hormones that are responsible for the onset of sexual differentiation in males and females and the development of secondary male physical characteristics such as a deep voice and facial hair. Though it is considered to be a “male” sex hormone, it is present in the blood of both men and women and is a precursor that can be converted by the body into more potent androgens, such as testosterone, or converted into the female hormone estrogen. This test measures the amount of androstenedione in the blood.

Androstenedione is produced by the ovaries in women, the testicles in men, and by the adrenal glands in both.

The pituitary hormone LH stimulates the release of androstenedione by the ovaries and testicles.
The pituitary hormone adrenocorticotropic hormone (ACTH) stimulates the release of androstenedione by the adrenal glands.

The level of androstenedione in the blood will vary during the day in a “diurnal pattern,” and it will vary during a woman’s menstrual cycle. Because of its origins, androstenedione can be useful as a marker of adrenal gland function, of androgen production, and of the function of the ovaries or testicles. An androstenedione test is often performed after results of other tests, such as testosterone or 17-hydroxyprogesterone, are found to be abnormal.

An excess level of androstenedione and other androgens can cause children to have sex organs that are not clearly male or female (ambiguous external genitalia), excess body hair (hirsutism), and abnormal menstrual periods in girls and precocious (early) puberty in girls and boys.

Adrenal tumors, ACTH-producing tumors, and adrenal hyperplasia can lead to the overproduction of androstenedione. Women with polycystic ovary syndrome (PCOS) may have higher levels of androstenedione as well. While elevated levels may not be noticed in adult men, they can lead to noticeable male physical characteristics (virilization) and a lack of monthly menstrual periods (amenorrhea) in females.

Common Questions

How is it used?

Androstenedione testing is used to evaluate the function of the adrenal gland and the ovaries or the testicles as well as the production of hormones related to the development of male sex organs and physical characteristics (androgens). It is most commonly used to determine the cause of symptoms of excess androgens in a woman who has an elevated testosterone level, along with a few other laboratory tests for this purpose.

An androstenedione level may be used to:

Evaluate adrenal gland function and to distinguish between androgen-secreting conditions that are caused by the adrenal glands from those that originate in the ovaries or testicles, if results of DHEAS and testosterone testing are abnormal
Help diagnose tumors in the outer layer (cortex) of the adrenal gland or tumors outside of the adrenal gland that secrete ACTH (ectopic) and separate these conditions from ovarian or testicular tumors and cancers
Diagnose congenital adrenal hyperplasia (CAH) and monitor CAH treatment, in addition to tests for testosterone and 17-hydroxyprogesterone, for example
Help diagnose polycystic ovarian syndrome (PCOS) and help rule out other causes of infertility, no monthly menstrual periods (amenorrhea), and excess body and facial hair (hirsutism) in women who have abnormal results on tests for DHEAS, testosterone, and other hormones such as FSH, LH, prolactin, and estrogen
Investigate and diagnose the cause of male physical characteristics (virilization) in young girls and early (precocious) puberty in young boys
Help determine the cause of delayed puberty and investigate suspected ovarian or testicular failure

When is it ordered?

Androstenedione may be ordered, along with or following other hormone tests, whenever excess (or, more rarely, deficient) androgen production is suspected and/or when a doctor wants to evaluate a person’s adrenal gland, ovarian, or testicular function.

It may be ordered when a female infant has external sex organs that are not clearly male or female (ambiguous external genitalia) or when a young girl develops male physical features that may be due to CAH or another condition associated with excess androgens.

Androstenedione may be measured when young boys show signs of precocious puberty – the development of an enlarged penis, muscularity, pubic hair, and/or a deeper voice – well before the age of normal puberty. It may also be ordered when puberty is delayed.

It may be measured when a woman has infertility or symptoms that can vary in severity and may include:

A deeper voice
Acne
Excess facial and/or body hair
Lack of or irregular menstruation
Male pattern baldness
Muscularity

Androstenedione is ordered periodically to help monitor glucocorticoid replacement therapy for CAH.

What does the test result mean?

A normal androstenedione level, along with other normal androgen levels and other normal adrenal tests, may indicate that the adrenal gland is functioning normally. However, an androstenedione level may be normal or elevated when an adrenal tumor or cancer is present, depending upon the hormones it is secreting. With polycystic ovarian syndrome, androstenedione may be elevated but may also be normal as this disorder is usually related to ovarian androgen production (primarily testosterone).

An elevated level of androstenedione indicates increased adrenal, ovarian or testicular production. Small fluctuations in concentration are usually normal. An increased level may indicate an adrenal tumor, adrenal cancer, adrenal hyperplasia, or congenital adrenal hyperplasia (CAH). An increased level is not diagnostic of a specific condition; it usually indicates the need for further testing to pinpoint the cause.

A low level of androstenedione may be due to adrenal gland dysfunction, adrenal insufficiency, or to ovarian or testicular failure.

In those with CAH who are being treated with glucocorticoid steroids, normal levels of androstenedione tend to indicate that treatment is effective in suppressing excess androgen production, while elevated levels indicate the need for treatment adjustment.

Is there anything else I should know?

If a radioimmunoassay method is used to measure androstenedione, then a radioactive scan performed within one week prior to testing may invalidate test results.

Androstenedione concentrations increase in boys undergoing puberty, for about two years prior to significant increases in testosterone. Androstenedione levels are not usually used to monitor this process.

Adrenal androstenedione is the major source of androgens for postmenopausal women.

Does everyone with elevated androstenedione have symptoms?

Not necessarily. The type and severity of symptoms will vary from person to person and will vary with age. Adult men have already developed masculine secondary sexual characteristics, so they will not typically experience symptoms with increased androstenedione.

How long will it take for androstenedione test results?

This depends on the laboratory performing the test. It is not a routine test and not all laboratories offer it. Your sample may need to be sent to a reference laboratory for testing. It may take several days for results to be available.

Is this test used for purposes other than detecting problems with the adrenal gland, ovaries or testicles?

Yes, the level of androstenedione (or “andro”) can be done as part of testing for sports doping. Androstenedione is sometimes taken illegally by athletes as a performance-enhancing drug. The body converts it to an anabolic steroid (a synthetic version of testosterone). It is intended to help build muscle, but like other anabolic steroids, it can interfere with bone growth, affect the heart, and in some cases cause liver damage.

View Sources

Sources Used in Current Review

Elhomsy, G. (Updated 2014 December 5). Androstenedione. Medscape Reference. Available online at http://emedicine.medscape.com/article/2088804-overview. Accessed March 2017.

(Reviewed 2015 June). Androstenedione, LC/MS/MS. Quest Diagnostics. Available online at http://www.questdiagnostics.com/testcenter/testguide.action?dc=Androstenedione&tabview=true. Accessed March 2017.

(2015 October 15). Performance-enhancing drugs: Know the risks. Mayo Clinic. Available online at http://www.mayoclinic.org/healthy-lifestyle/fitness/in-depth/performance-enhancing-drugs/art-20046134. Accessed March 2017.

Koch, C. (2015 December 16). Androgen Insensitivity Syndrome. Medscape Reference. Available online at http://emedicine.medscape.com/article/924996-overview. Accessed March 2017.

Meikle, A. (Updated 2016 August). Congenital Adrenal Hyperplasia – CAH. ARUP Consult. Available online at http://www.arupconsult.com/Topics/CAH.html?client_ID=LTD. Accessed March 2017.

Straseski, J. A. (2017 February). Polycistic ovary syndrome (PCOS). ARUP Laboratories. Available online at https://arupconsult.com/content/polycystic-ovarian-syndrome. Accessed March 2017.

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Georgopoulos NA, Papadakis E, Armeni AK, Katsikis I, Roupas ND, Panidis D). Elevated serum androstenedione is associated with a more severe phenotype in women with polycystic ovary syndrome (PCOS). Hormones (Athens). 2014 Apr-Jun;13(2):213-21.

Sources Used in Previous Reviews

Elhomsy, G. (Updated 2012 September 12). Androstenedione. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/2088804-overview. Accessed May 2013.

(© 1995–2013). Androstenedione, Serum. Mayo Clinic Mayo Medical Laboratories [On-line information]. Available online at http://www.mayomedicallaboratories.com/test-catalog/Overview/9709. Accessed May 2013.

(2009 August). Androstenedione and Dehydroepiandrosterone in Serum by LC-MS/MS. ARUP Laboratories [On-line information]. Available online through http://www.aruplab.com. Accessed May 2013.

Wilson, T. (Updated 2013 June 13). Congenital Adrenal Hyperplasia. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/919218-overview. Accessed May 2013.

Meikle, A. (Updated 2013 January). Congenital Adrenal Hyperplasia – CAH. ARUP Consult [On-line information]. Available online at http://www.arupconsult.com/Topics/CAH.html?client_ID=LTD. Accessed May 2013.

Koch, C. (2012 July 12). Androgen Insensitivity Syndrome. Medscape Reference [On-line information]. Available online at http://emedicine.medscape.com/article/924996-overview. Accessed May 2013.

Higgins, J. et. al. (2012). Androgen Abuse and Increased Cardiac Risk. Medscape Today News from South Med J. v 105 (12):670-674 [On-line information]. Available online at http://www.medscape.com/viewarticle/775869. Accessed May 2013.

Lebbe, M. et. al. (2012). Androgen Replacement Therapy in Women. Medscape Reference from Expert Rev Endocrinol Metab. v7 (5):515-529. [On-line information]. Available online at http://www.medscape.com/viewarticle/773774_3. Accessed May 2013.

(Updated 2011 May 23). Androgen. Healthy Women [On-line information]. Available online at http://www.healthywomen.org/condition/androgen. Accessed May 2013.

Marx, T. and Mehta, A. (2003 January). Polycystic ovary syndrome: Pathogenesis and treatment over the short and long term. Cleveland Clinic Journal of Medicine v 70 (1) [On-line information]. Available online at http://www.ccjm.org/content/70/1/31.full.pdf. Accessed May 2013.

Rivkees, S. (© 2007-2011). Congenital Adrenal Hyperplasia. Monitoring Treatment of Children. CARES Foundation [On-line information]. Available online at http://www.caresfoundation.org/productcart/pc/children_cah.html. Accessed May 2013.

(Revised 2012). Hirsutism and Polycystic Ovary Syndrome (PCOS), A Guide for Patients. American Society for Reproductive Medicine [On-line information]. Available online through http://www.asrm.org. Accessed May 2013.

Dowshen, S. (Reviewed 2010 October). Steroids. KidsHealth from Nemours [On-line information]. Available online at http://kidshealth.org/parent/emotions/behavior/steroids.html. Accessed May 2013.

Mayo Clinic staff (2010 December 22). Performance-enhancing drugs and teen athletes. Mayo Clinic [On-line information]. Available online at http://www.mayoclinic.com/print/performance-enhancing-drugs/SM00045/METHOD=print. Accessed May 2013.

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